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Case study: RNA-seq data analysis – HarvardX- EDX

0-6 Month Online
Intermediate Contact Institute
Online Business Analytics
Online Self Paced 1801


[su_tab title = “Description”]

In the PH525 case studies, we will explore the data analysis of an experimental protocol in depth, using various open source software, including R and Bioconductor. We will explain how to start with raw data, and perform the standard processing and normalization steps to get to the point where one can investigate relevant biological questions.Throughout the case studies, we will make use of exploratory plots to get a general overview of the shape of the data and the result of the experiment.


[su_tab title = “Program Structure”]

We will learn the basic steps for analyzing an RNA-seq dataset for an organism with a well-defined genome and curated gene annotation. This will include read alignment, inferring presence of isoforms, counting reads in genes, exploring sample distances, differential expression analysis, and creating visual summaries.


2 weeks

6 hours per week

Important Date

Contact Institute

Registration open through 4 May 2015

Full time/Part time

Part time


[su_tab title = “Eligibility”]

  • Interested candidates must have knowledge about PH525.3x, PH525.4x


[su_tab title =”Tools”]

  • R


[su_tab title = “Faculty”]

  • Rafael Irizarry
  • Michael Love


[su_tab title = “Contact”]

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